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GC07

Custom Molecular, Sequencing & Bioinformatics Services

Custom molecular, sequencing and bioinformatics services, including gene synthesis, cloning, NGS and analytical services — scoped and quoted per project.

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Cat. No.ProductPackYield / specStoragePrice*
GLT-GC07-001 Custom Gene Synthesis Service of 120 bp - 500 bp Per construct ₹25,000
GLT-GC07-002 Custom Gene Synthesis Service of 501 bp - 1000 bp Per construct ₹42 / bp
GLT-GC07-003 Custom Gene Synthesis Service of 1001 bp - 2000 bp Per construct ₹48 / bp
GLT-GC07-004 Custom Gene Synthesis Service of 2001 bp - 4000 bp Per construct ₹58 / bp
GLT-GC07-005 Custom Gene Synthesis Service of ≥ 4 kb Per construct On request
GLT-GC07-006 Codon optimization of the gene of interest Per construct ₹10,000
GLT-GC07-007 Mitochondrial DNA extraction service (from tissues, blood, cultures) up to 2-20ug Samples: Frozen tissue, cells & blood samples. Per Sample ₹25,000
GLT-GC07-008 Genomic DNA extraction service (from tissues, blood, cultures) up to 20ug Samples: Frozen tissue, cells & blood samples. Per Sample ₹12,500
GLT-GC07-009 Genomic DNA extraction service (from tissues, blood, cultures) up to 20ug Samples: Frozen tissue, cells & blood samples. up to 10 samples ₹35,700
GLT-GC07-010 Genomic DNA isolation from plant samples. Samples: Frozen plant samples. Per Sample ₹12,500
GLT-GC07-011 Genomic DNA isolation from plant samples. Samples: Frozen plant samples. up to 10 samples ₹37,890
GLT-GC07-012 Custom plasmid isolation service (Deliverable: up to 10 ug pDNA) Per Sample ₹10,000
GLT-GC07-013 Custom plasmid isolation service (Deliverable: up to 200 ug pDNA) Per Sample ₹20,000
GLT-GC07-014 Custom plasmid isolation service (Deliverable: up to 1 mg pDNA) Per Sample ₹40,000
GLT-GC07-017 PCR Optimization and quality check through Agarose gel electrophoresis Per Target ₹5,500
GLT-GC07-018 PCR Optimization and quality check through Agarose gel electrophoresis up to 10 Targets ₹15,000
GLT-GC07-019 Total RNA Extraction service and quality check by Agarose gel electrophoresis (Phenolic method) Per Sample ₹15,000
GLT-GC07-020 Total RNA Extraction service and quality check by Agarose gel electrophoresis (phenolic method) up to 10 samples ₹52,000
GLT-GC07-021 Total RNA Extraction service and quality check by Agarose gel electrophoresis (non-phenolic method) Per Sample ₹18,000
GLT-GC07-022 Total RNA Extraction service and quality check by Agarose gel electrophoresis (non-phenolic method) up to 10 samples ₹60,000
GLT-GC07-023 Total RNA Extraction service and quality check by MOPS (Phenolic method) Per Sample ₹20,000
GLT-GC07-024 Total RNA Extraction service and quality check by MOPS (phenolic method) up to 10 samples ₹57,000
GLT-GC07-025 Total RNA Extraction service and quality check by MOPS (non-phenolic method) Per Sample ₹23,000
GLT-GC07-026 Total RNA Extraction service and quality check by MOPS (non-phenolic method) up to 10 samples ₹65,000
GLT-GC07-027 cDNA synthesis from total RNA/ mRNA; Reverse transcription experiment PCR: First strand cDNA synthesis by Oligo dT or random hexamer, includes primer synthesis of house keeping gene Per Sample ₹25,500
GLT-GC07-028 cDNA synthesis from total RNA/ mRNA Reverse transcription PCR: First strand cDNA synthesis by Oligo dT or random hexamer, includes primer synthesis of house keeping gene up to 10 samples ₹98,000
GLT-GC07-029 Quantitative real time PCR by SYBR green method (up to 24 reactions) Per Test ₹25,000
GLT-GC07-031 Quantitative real time PCR by TAQMAN chemistry (up to 24 reactions) Per Test ₹35,000
GLT-GC07-033 Real time data analysis (fold increase, relative expression, absolute quantification) Per Analysis ₹10,000
GLT-GC07-034 Primer designing for standard PCR Per Primer Set ₹500
GLT-GC07-035 Primer designing for real time qPCR Per Primer Set ₹1,000
GLT-GC07-036 Primer and TaqMan probe designing for real time qPCR Per Primer Set ₹1,500
GLT-GC07-037 LAMP Primer designing and synthesis Per Primer Set ₹50,000
GLT-GC07-038 Molecular cloning of custom DNA fragment, includes: Restriction digestion of customer-provided vector and insert with appropriate restriction enzymes; treatment with phosphatases & PNK as required and gel purification; Ligation of digested fragments to generate desired vector clone; screening and selection of positive clones by PCR and/or restriction digestion; Cloning confirmation by 'vector: insert end-joint' sequencing; MiniPrep for selected plasmid- ~2mg of isolated plasmid DNA will be provided. Per Cloning ₹35,000
GLT-GC07-039 Introduction of point mutation by Site directed Mutagenesis; includes : Design and synthesis of mutagenesis primers, PCR amplification, Confirmation of desired mutations by DNA sequencing, Delivery of purified plasmid DNA construct or its glycerol stock. (single point or multiple point mutations within 6 bases) Per SDM Reaction ₹45,000
GLT-GC07-040 Preparation of deletion mutant (≥10 bp from wild-type) construct; includes: Design and synthesis of primers, PCR amplification, Confirmation of desired mutations by DNA sequencing, Delivery of purified plasmid DNA construct or its glycerol stock. (30% extra charge above the unit price will be applicable in case of amplicon size ≥10 kb) Per Construct ₹65,000
GLT-GC07-041 Custom protein expression analysis by western blot Includes: Protein extraction; SDS-PAGE, Blotting and immunoprobing, (protein specific Antibody have to be provided by the customer; extra charge will be applicable if our company provides antibody) and detection (chemiluminescence detection and chromogenic detection). Per Blot ₹65,000
GLT-GC07-042 Bulk Protein expression and purification, in bacterial host includes: Cloning of Target gene in the expression vector, Expression analysis in the host, purification of the protein and characterization by SDS-PAGE. 1-5 mg bulk protein will be supplied in solution or lyophilized form. Per Protein ₹8,50,000
GLT-GC07-043 Custom protein expression analysis by SDS PAGE and Coomassie stain (E.Coli expression system) Per Gel Analysis ₹19,800
GLT-GC07-044 Standard 2-Dimensional SDS-PAGE, electronic photo and file will be provided Per Gel Analysis ₹1,50,000
GLT-GC07-045 Standard 2-Dimensional SDS-PAGE, electronic photo and file will be provided Per Two Gel Analysis ₹2,50,000
GLT-GC07-046 Custom Peptide Synthesis (Price per amino acid for peptide of 6-30 amino acids*) Per Amino Acid On request
GLT-GC07-048 Polyclonal antibody development up to 1 mg Includes: QC check, control sera isolation, immunization, total sera collection, antibody titre checking by ELISA supply of pre-immune and immunised sera up to 1ml to the customer Per Antibody ₹1,10,000
GLT-GC07-049 Affinity purification of Antibody by protein A/G column, 1mg ₹65,000
GLT-GC07-050 QC check (IP, WB, ELISA) On request
GLT-GC07-051 Western Blot-Check ₹20,000
GLT-GC07-054 Sanger sequencing (500bp read) Per stretch ₹1,200
GLT-GC07-055 Sequencing Native bacterial Plasmids Per bp ₹8
GLT-GC07-056 SNP detection and data analysis Per 500 bases ₹7,500
GLT-GC07-057 SNP detection and data analysis (Charges apply, if PCR fails even after repeated attempts) Per 500 bases ₹7,500
GLT-GC07-058 16S rDNA Sequencing with gene-specific primers Per isolate ₹15,000
GLT-GC07-059 16S rDNA Sequencing with universal primers Per isolate ₹12,000
GLT-GC07-060 18S rDNA Sequencing with gene-specific primers Per isolate ₹15,000
GLT-GC07-061 18S rDNA Sequencing with universal primers Per isolate ₹12,000
GLT-GC07-062 ITS sequencing with universal primers Per Isolate ₹15,000
GLT-GC07-063 ITS sequencing with gene-specific primers Per Isolate ₹12,000
GLT-GC07-064 Unknown gene sequencing (from reported homologous sequences, 5' & 3' RACE, up to 2 Kb) per sample ₹5,00,000
GLT-GC07-065 Plant Genotyping Services for pathogen detection per disease On request
GLT-GC07-066 AFLP Fragment Analysis: Gel based polymorphism analyses within inter/intra species populations, varieties, strains, etc. (Primer design and synthesis charges extra) one sample/ marker ₹2,500
GLT-GC07-067 Genotyping by RAPD Analysis: Gel based polymorphism analysis within closely related samples as well as Primer information, agarose gel photo, etc. provided one sample/ marker ₹2,500
GLT-GC07-068 Genotyping by SSR/ISSR/STR analysis; Agarose gel based polymorphism report; Oligo design and synthesis & Bioinformaics analysis charged separately one sample/ marker ₹2,500
GLT-GC07-069 Multi-Locus Sequencing Typing (MLST): Once the sample information is available genotyping can be initiated; From genomic DNA isolation to PCR amplification and sequencing with customer provided oligo information; Chromatogram and analysis will be provided Per Specimen ₹50,000
GLT-GC07-070 Mitochondrial Sequencing to study human diseases such as diabetes, certain cancers, and mechanisms of aging and population genetics and biodiversity assessments Per target ₹6,500
GLT-GC07-071 Setup charges includes: (1) Project design, (2) Primer synthesis (desalted standard), (3)PCR optimization, (4) DNA isolation, purification, quality check per project ₹2,00,000
GLT-GC07-072 PCR Amplification optimization and quality confirmation per amplification ( Min 1000 amplifications) ₹95
GLT-GC07-073 Bioanalyzer-based analysis per sample analysis ₹20,000
GLT-GC07-074 capillary genetic analyser-based fragment analysis- (Modified primer will be provided on additional standard market price) per 96 well plate ₹1,80,000
GLT-GC07-075 Sequencing of polymorphic bands with both end sanger sequencing per sample/ per band ₹2,500
GLT-GC07-076 Bioinformatics analysis: Genetic Profiling of germplasm for geographical; Identification and biodiversity analysis; Germplasm Mapping analysis; QTL analysis (morphological data provided by customer); Biodiversity analysis; Phylogenetic tree construction; Clustering of population Statistical analysis for population study; Parental evaluation; Marker assisted profiling per project ₹3,00,000
GLT-GC07-077 Setup charges: For Large population analysis (Upto 100 samples) Includes Project design; Primer synthesis (desalted standard); PCR optimization per project ₹3,00,000
GLT-GC07-078 Custom quantitative real time PCR based diagnostic kit development ₹22,55,690
GLT-GC07-079 DNA Barcoding Service (per two targets) Each Sample ₹18,985
GLT-GC07-080 HPLC based Small Molecule Quantification per sample ₹1,50,000
GLT-GC07-081 HPLC-LCMS based Metabolomics Analysis per sample On request
GLT-GC07-082 Plant Profiling (HPLC based) per sample On request
GLT-GC07-083 Cell Profiling (HPLC based) per sample On request
GLT-GC07-084 Analytical Service charges for Mass Spectrometry per sample ₹5,28,000
GLT-GC07-085 Analytical Service charges for LCMS per sample On request
GLT-GC07-086 Analytical Service charges for HPLC per sample ₹2,32,000
GLT-GC07-087 Preparative Service charges for HPLC per sample ₹2,50,000
GLT-GC07-088 Analytical Service charges for 1H NMR (CDCl3), 500 MHz per sample ₹8,28,000
GLT-GC07-089 Analytical Service charges for 1H NMR (DMSO_d6), 500 MHz per sample ₹8,36,000
GLT-GC07-090 Analytical Service charges for 1H NMR (MeOD_d4), 500 MHz per sample ₹83,600
GLT-GC07-091 Analytical Service charges for 1H NMR (D2O), 500 MHz per sample ₹83,600
GLT-GC07-092 Analytical Service charges for 13C NMR(CDCl3), 500 MHz, up to 1hr. per sample ₹77,200
GLT-GC07-093 Analytical Service charges for 13C NMR(DMSO_d6), 500 MHz, up to 1hr. per sample ₹88,000
GLT-GC07-094 Analytical Service charges for 13C NMR(MeOD_d4), 500 MHz, up to 1hr. per sample ₹88,000
GLT-GC07-095 Analytical Service charges for 13C NMR(D2O), 500 MHz, up to 1hr. per sample ₹88,000
GLT-GC07-096 Draft sequencing of bacteria/ virus/ fungi by mechanical shearing process from purified genomic DNA 10 Mb to 100 Mb On request
GLT-GC07-098 Draft sequencing of bacteria/ virus/ fungi by mechanical shearing process from purified genomic DNA 100 Mb to 500 Mb On request
GLT-GC07-100 Draft sequencing of bacteria/ virus/ fungi by mechanical shearing process from purified genomic DNA above 500 Mb On request
GLT-GC07-102 Draft sequencing of bacteria/ virus/ fungi by enzymatical shearing process from purified genomic DNA 10 Mb to 100 Mb On request
GLT-GC07-104 Draft sequencing of bacteria/ virus/ fungi by enzymatical shearing process from purified genomic DNA 100 Mb to 500 Mb On request
GLT-GC07-106 Draft sequencing of bacteria/ virus/ fungi by enzymatical shearing process from purified genomic DNA above 500 Mb On request
GLT-GC07-108 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by mechanical shearing process from purified genomic DNA 10 Mb to 100 Mb On request
GLT-GC07-110 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by mechanical shearing process from purified genomic DNA 100 Mb to 500 Mb On request
GLT-GC07-112 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by mechanical shearing process from purified genomic DNA above 500 Mb On request
GLT-GC07-114 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by enzymatical shearing process from purified genomic DNA 10 Mb to 100 Mb On request
GLT-GC07-116 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by enzymatical shearing process from purified genomic DNA 100 Mb to 500 Mb On request
GLT-GC07-118 Reference assisted sequencing or targeted sequencing of bacteria / virus/ fungi by enzymatical shearing process from purified genomic DNA above 500 Mb On request

* All prices are in Indian Rupees (INR), exclusive of GST and freight, and are subject to change without notice.