At Grace Lifetech, our Single B Cell Sorting Service offers a cutting-edge approach to the rapid and efficient production of highly specific monoclonal antibodies. By isolating individual B cells from immunized animals or humans, we can directly access the genetic information of antibodies produced during a natural immune response. This advanced method enables us to produce monoclonal antibodies with unparalleled speed, precision, and diversity, making it ideal for therapeutic, diagnostic, and research applications.

Why Choose Single B Cell Sorting?

1. Fast and Efficient Antibody Discovery
   Single B cell sorting significantly accelerates the timeline for monoclonal antibody discovery. Unlike traditional hybridoma or phage display methods, which can take months, single B cell sorting allows for rapid identification of antigen-specific B cells and antibody gene sequences in a matter of weeks.
2. High-Affinity Antibodies
   By isolating B cells directly from the immune system, this method yields naturally matured antibodies with high affinity and specificity. These antibodies are ideal for applications requiring precise target recognition, such as therapeutic antibody development, diagnostics, and biomarker detection.
3. Broad Epitope Coverage
   Single B cell sorting allows for the identification of antibodies that recognize a wide range of epitopes, including rare or unique epitopes that may be difficult to detect with traditional approaches. This enhances the ability to generate antibodies against challenging targets, including viruses, cancer cells, and complex antigens.
4. Human and Animal B Cell Sorting
   Our platform enables the isolation of B cells from both human donors and a variety of immunized animal species (e.g., mice, rabbits, or non-human primates). This flexibility allows for the generation of human or animal-derived antibodies depending on your project’s specific needs.
5. Direct Access to In Vivo Antibody Repertoires
   Single B cell sorting provides direct access to the natural antibody repertoires generated by the immune system in response to immunization or infection. This results in highly functional antibodies with enhanced biological activity and therapeutic potential.

Our Single B Cell Sorting Workflow

1. Antigen Design and Immunization
   We begin by designing or optimizing the antigen to ensure a robust immune response. If working with animal models, we immunize the animals (mouse, rabbit, etc.) with your antigen of interest. For human samples, we collect peripheral blood mononuclear cells (PBMCs) from immunized or infected individuals, or patients with naturally occurring antibodies.
2. Single B Cell Isolation and Sorting
   Using advanced flow cytometry and fluorescence-activated cell sorting (FACS), we isolate individual B cells that express antigen-specific antibodies. By labeling the target antigen with a fluorescent marker, we can identify and sort the B cells that produce antibodies with high affinity for your antigen of interest.
3. Antibody Gene Amplification and Cloning
   After isolating individual B cells, we recover the genetic information for both the heavy and light chains of the antibody by amplifying the antibody genes using RT-PCR. We then clone these genes into expression vectors for recombinant production in mammalian or other suitable expression systems.
4. Antibody Expression and Screening
   Once cloned, we express the antibodies recombinantly and perform functional screening to assess their binding affinity, specificity, and biological activity. We use high-throughput assays, including ELISA, flow cytometry, and functional assays, to identify the best-performing antibodies.
5. Antibody Production and Purification
   After screening, we scale up the production of the top-performing antibodies. We use advanced purification methods, such as protein A/G affinity chromatography, to deliver high-quality, pure antibodies that are ready for your downstream applications.
6. Characterization and Quality Control
   Each antibody undergoes comprehensive characterization and quality control, including isotyping, binding affinity measurement, and functional validation. We ensure that all antibodies meet your desired specifications for purity, yield, and performance.

Applications of Single B Cell Sorting

• Therapeutic Antibody Development
  Single B cell sorting is ideal for identifying antibodies with therapeutic potential. By isolating naturally high-affinity antibodies from immunized animals or human donors, this method accelerates the discovery of lead candidates for cancer immunotherapy, infectious disease treatment, autoimmune disease management, and more.
• Vaccine Development
  Generate high-affinity antibodies against viral or bacterial pathogens, which can be used to develop new vaccines or study immune responses to infectious agents. Single B cell sorting allows for the rapid identification of neutralizing antibodies that target viral entry proteins or other essential epitopes.
• Diagnostic Antibody Generation
  Produce monoclonal antibodies for use in diagnostic assays, such as ELISA, lateral flow tests, and biosensor platforms. Single B cell sorting enables the rapid generation of highly specific antibodies that can detect disease biomarkers or pathogens with high sensitivity.
• Rare Epitope Identification
  Single B cell sorting is particularly effective for identifying antibodies that bind to rare or hidden epitopes, making it a powerful tool for generating novel antibodies against complex or difficult-to-target antigens.

Advantages of Our Single B Cell Sorting Service

• Rapid Antibody Discovery
  This technology significantly reduces the time required for monoclonal antibody generation, making it possible to discover high-affinity antibodies in just weeks.
• High Specificity and Affinity
  By isolating B cells directly from immunized animals or human donors, we ensure the production of antibodies with naturally high affinity and specificity, minimizing the need for further optimization.
• Tailored Solutions
  We offer fully customizable services, from antigen design to final antibody production. Whether you need therapeutic-grade antibodies or research reagents, our team tailors the process to meet your specific needs.
• Scalable Production
  Once your antibody is identified, we offer scalable production solutions, from small research quantities to large-scale commercial production, ensuring a seamless transition from discovery to application.
• Comprehensive Support
  From initial consultation through antibody delivery, our team provides expert support and guidance at every step of the process to ensure your project’s success.

Why Partner with Us?

• State-of-the-Art Technology: We utilize advanced flow cytometry and single-cell technologies to rapidly isolate and identify antigen-specific B cells with precision and accuracy.
• Experienced Team: Our team of immunologists and antibody engineers has extensive expertise in B cell sorting, antibody production, and therapeutic development.
• Customized Approach: Every project is unique, and we work closely with you to design a customized solution that meets your project’s goals and timelines.
• High-Quality Results: We deliver high-affinity, high-specificity antibodies backed by comprehensive quality control and rigorous testing.

Contact us today to learn how our Single B Cell Sorting Service can accelerate your antibody discovery and development projects with precision, speed, and reliability. Let us help you unlock the full potential of monoclonal antibodies for your research or therapeutic needs.