Our Cell-Free Protein Expression Service delivers a rapid, flexible, and scalable solution for producing high-quality proteins without the limitations of living cells. Leveraging advanced cell-free systems, we enable efficient synthesis of proteins for applications in structural biology, drug discovery, functional assays, and more. Ideal for projects requiring high-throughput, difficult-to-express proteins, or post-translational modifications, our service is designed to meet the demands of research and development in various fields.

Why Choose Cell-Free Protein Expression?

1. Rapid Protein Production
   Cell-free systems allow for the quick production of proteins, with expression complete in just a few hours. This speed is ideal for high-throughput applications, accelerating workflows for research, assay development, and drug discovery.

2. Flexibility with Complex Proteins
   Cell-free expression is highly adaptable, allowing production of proteins that are challenging to express in traditional systems, including membrane proteins, toxic proteins, and multi-subunit complexes. This flexibility makes cell-free systems an excellent choice for challenging proteins or those requiring specific modifications.

3. Scalable and High-Throughput
   Our cell-free platform supports both small-scale expression for rapid screening and large-scale synthesis for extensive studies. This scalability, combined with the ability to express multiple constructs simultaneously, offers an efficient approach for protein libraries or variant screenings.

4. Enhanced Labeling and Modifications
   Cell-free expression systems can incorporate non-standard amino acids and isotopic labels, enabling advanced studies in protein structure, function, and interaction. These systems also allow for custom post-translational modifications, such as phosphorylation and glycosylation, to support specific experimental needs.

5. Minimal Setup and Faster Results
   Without the need for live cell cultures, cell-free expression reduces time, cost, and complexity associated with maintaining cells, enabling direct translation from DNA to functional protein in hours rather than days.

Our Cell-Free Protein Expression Workflow

1. DNA Template Preparation
   Protein production begins with template DNA, which can be provided by you or synthesized in-house. Templates can be plasmids or linear DNA, making it simple to screen a variety of constructs or mutations.

2. Cell-Free System Selection
   Based on your protein type and intended application, we select the optimal cell-free system, such as E. coli lysate, wheat germ, insect, or mammalian cell extracts. Each system offers specific benefits, from high-yield bacterial systems to eukaryotic systems that support complex folding and modifications.

3. Protein Expression Optimization
   We optimize reaction conditions, including temperature, duration, and reagent concentrations, to maximize yield and functionality for your specific protein. Additional optimization ensures proteins are expressed with minimal degradation or aggregation.

4. Purification and Isolation
   After expression, we employ high-quality purification techniques, such as affinity chromatography, size exclusion, or ion exchange chromatography, to ensure high-purity proteins. Our purification methods can be customized based on protein requirements, purity levels, and downstream applications.

5. Labeling and Modification
   If required, we incorporate specialized labeling, such as isotopic labels (^15N, ^13C) or fluorescent tags, to facilitate applications in NMR, mass spectrometry, or fluorescence-based assays. Post-translational modifications are also available to match your protein’s native form.

6. Quality Control and Validation
   Every protein batch undergoes rigorous quality control, including SDS-PAGE, Western blotting, mass spectrometry, and functional assays to ensure purity, activity, and yield meet your specifications.

7. Protein Delivery and Project Support
   Once production is complete, we deliver your protein in the desired concentration and format. Our team provides ongoing technical support to ensure your protein performs optimally in your experiments.

Applications of Cell-Free Protein Expression

• Structural Biology and Protein Crystallography
  Our service enables rapid production of proteins, including labeled variants, for studies in NMR, X-ray crystallography, and cryo-EM, supporting advanced structural analysis of proteins and protein complexes.

• Drug Discovery and Screening
  Cell-free expression allows for high-throughput screening of protein targets, binding studies, and inhibitor testing. With quick turnaround and scalability, our service supports both early-stage discovery and lead optimization.

• Functional and Biochemical Assays
  We provide proteins ready for use in functional assays, including enzymatic, binding, and inhibition studies. The ability to incorporate custom labels and modifications further enhances the utility of proteins in diverse assay formats.

• Antibody Production and Epitope Mapping
  Cell-free systems allow rapid expression of protein fragments and mutants, making them ideal for epitope mapping, antibody production, and antibody validation.

• Synthetic Biology and Protein Engineering
  With cell-free expression, we support synthetic biology applications such as protein engineering, variant library screening, and the development of novel protein-based materials.

Advantages of Our Cell-Free Protein Expression Service

• Fast Turnaround
  Expressing proteins within hours, our cell-free system significantly shortens project timelines, allowing you to quickly access high-quality proteins for your studies.

• High Customizability
  Our platform accommodates a range of expression systems and labeling options, tailoring protein production to meet the specific needs of your project.

• High-Yield, High-Purity Proteins
  We employ optimized protocols and advanced purification techniques to deliver proteins that meet the highest standards of purity, functionality, and yield.

• Flexible Scale
  From small-scale expression for assay development to larger quantities for extensive studies, we offer scalable solutions tailored to your project’s demands.

Why Partner with Us?

• Expertise in Protein Engineering: Our team has extensive experience in protein engineering, expression optimization, and biochemical assays, ensuring your proteins meet performance requirements.
• State-of-the-Art Technology: We use the latest cell-free expression systems and purification techniques to deliver proteins of the highest quality and reliability.
• Project Flexibility: Our customizable workflows and flexible scale allow us to adapt to your project’s unique requirements, whether you need labeled proteins, difficult-to-express targets, or large protein quantities.
• Comprehensive Support: From design to delivery, we provide comprehensive support at every stage, ensuring your proteins are ready for immediate use in your applications.

Contact us today to discuss how our Cell-Free Protein Expression Service can advance your research, streamline your workflows, and deliver high-quality proteins tailored to your project’s needs. Let us be your trusted partner for innovative, efficient, and reliable protein expression.