At Grace Lifetech, we offer state-of-the-art Single B Cell Screening Services using Fluorescence-Activated Cell Sorting (FACS), an advanced technique designed to rapidly identify and isolate antigen-specific B cells. This powerful approach accelerates the discovery of high-affinity monoclonal antibodies directly from the immune repertoire of humans or immunized animals, providing faster, more accurate, and highly efficient antibody discovery.

Why Choose FACS-Based Single B Cell Screening?

1. Rapid and Efficient Antibody Discovery
   FACS-based single B cell screening allows for the direct identification and isolation of individual B cells that produce antibodies specific to your target antigen. This method significantly reduces the time required to generate high-quality monoclonal antibodies compared to traditional methods, such as hybridoma technology or phage display.
2. High Specificity and Affinity
   Single B cell screening enables the isolation of antibodies that are naturally produced during an immune response, resulting in antibodies with high specificity and affinity. These antibodies can be used in a variety of applications, including diagnostics, therapeutics, and research, where precise target recognition is critical.
3. Direct Access to Functional Antibodies
   Unlike other antibody discovery methods, single B cell screening captures antibodies in their naturally produced form, offering direct access to fully functional antibodies. This allows for the rapid identification of biologically relevant antibodies with strong binding affinity and potent functional activity.
4. Single-Cell Precision
   FACS provides the ability to analyze and sort individual B cells based on specific markers and antigen-binding properties. This single-cell resolution ensures that only the best-performing B cells are selected for downstream cloning and antibody production, resulting in a highly focused and efficient antibody discovery process.

Our FACS-Based Single B Cell Screening Workflow

1. Antigen Preparation and Immunization
   We start by designing or optimizing the antigen to elicit a robust immune response. Whether you provide your antigen or we design it in-house, we ensure it is tailored to stimulate a targeted immune response. If working with animals, we immunize them with your antigen, or we collect samples from human donors (e.g., peripheral blood mononuclear cells (PBMCs) from immunized or infected individuals).
2. B Cell Isolation
   Once the immune response is established, we isolate B cells from the collected samples using advanced methods. These B cells are then prepared for FACS, where we focus on antigen-specific B cells.
3. Fluorescence-Activated Cell Sorting (FACS)
   Using FACS technology, we label your antigen of interest with a fluorescent marker, allowing us to screen for and sort B cells that express antigen-specific antibodies. FACS precisely analyzes individual B cells based on surface markers and fluorescence intensity, identifying cells that are highly reactive to the target antigen. This process isolates the top-performing B cells, which are then used for further analysis.
4. Antibody Gene Amplification and Cloning
   After the antigen-specific B cells are sorted, we extract the genetic material encoding the antibody’s heavy and light chains. Using RT-PCR, we amplify these antibody genes and clone them into expression vectors, enabling recombinant production of the antibodies in mammalian cell systems.
5. Antibody Expression and Screening
   Once the antibody genes are cloned, we express the antibodies in a suitable expression system and screen them using assays such as ELISA, flow cytometry, and other functional assays. This allows us to evaluate their binding affinity, specificity, and functional activity against the target antigen.
6. Antibody Production and Purification
   Following the screening, we produce the selected antibodies at scale and purify them using advanced techniques such as protein A/G affinity chromatography. This ensures that you receive high-quality, functional monoclonal antibodies ready for downstream applications.

Applications of Single B Cell Screening (FACS)

• Therapeutic Antibody Discovery
  FACS-based single B cell screening is highly effective for the rapid discovery of therapeutic antibodies. This method accelerates the identification of lead candidates for cancer immunotherapy, infectious disease treatment, autoimmune disorders, and other therapeutic areas by directly isolating high-affinity antibodies from immunized animals or human donors.
• Vaccine Development
  Single B cell screening provides a powerful tool for identifying neutralizing antibodies against viral or bacterial pathogens, aiding in the development of vaccines. This method allows for the rapid detection of antibodies that target critical epitopes required for pathogen neutralization.
• Diagnostic Antibody Generation
  Create highly specific antibodies for diagnostic applications, including ELISA, lateral flow tests, and biosensors. FACS allows for the rapid generation of antibodies that bind to disease biomarkers or pathogens with high sensitivity, improving diagnostic accuracy.
• Rare Epitope Discovery
  FACS-based single B cell sorting can isolate antibodies that recognize rare or hidden epitopes, making it ideal for generating novel antibodies against difficult-to-target antigens, such as viruses, cancer cells, or post-translational modifications.

Advantages of Our FACS-Based Single B Cell Screening Service

• Speed
  FACS dramatically reduces the time required to discover and produce monoclonal antibodies, allowing for the identification of antigen-specific B cells in just a few weeks.
• High Sensitivity
  FACS-based sorting provides high sensitivity, enabling the isolation of rare B cells that produce high-affinity antibodies, even in complex biological samples.
• Single-Cell Precision
  The single-cell resolution of FACS ensures the selection of individual B cells based on highly specific criteria, leading to more focused and efficient antibody discovery.
• Fully Functional Antibodies
  By directly isolating B cells that are actively producing antibodies, we obtain fully functional antibodies without the need for further optimization or affinity maturation.

Why Partner with Us?

• Cutting-Edge Technology: We use the latest FACS technology to ensure high precision and efficiency in identifying antigen-specific B cells.
• Expert Team: Our team of immunologists and antibody engineers has extensive experience in single B cell screening and therapeutic antibody discovery.
• Tailored Solutions: Every project is unique, and we customize our services to meet your specific research or development goals, from antigen design to final antibody production.
• Comprehensive Support: From initial consultation through antibody production and quality control, we provide expert guidance and transparent communication at every step of the process.

Contact us today to learn how our Single B Cell Screening Service (FACS) can accelerate your antibody discovery process and help you achieve your research, diagnostic, or therapeutic goals with high-speed, high-affinity antibodies.