Our Single B Cell Screening Service offers a robust, cutting-edge solution for discovering high-affinity monoclonal antibodies directly from individual B cells. By isolating and analyzing single B cells from immunized animals or human samples, we provide highly specific and efficient antibody discovery for therapeutic, diagnostic, and research applications. This service allows rapid access to functional antibodies that represent the natural immune response, delivering superior specificity, affinity, and functionality.

Why Choose Single B Cell Screening?

1. Rapid Antibody Discovery
   Single B cell screening enables the direct identification of antigen-specific B cells, significantly accelerating timelines for antibody discovery. Compared to traditional methods like hybridoma or phage display, single B cell screening delivers high-affinity antibodies in weeks, ideal for fast-paced research and development.

2. High Specificity and Affinity
   By isolating B cells that naturally produce antibodies in response to an antigen, we access antibodies with high binding affinity and specificity. These high-quality antibodies are suitable for therapeutic, diagnostic, and research applications that demand precise target recognition.

3. Broad Epitope Coverage
   Single B cell screening enables the discovery of antibodies that bind to diverse or rare epitopes, making it possible to generate antibodies against complex targets, including pathogens, cancer cells, and challenging antigens that traditional methods may struggle to detect.

4. Human and Animal B Cell Sources
   Our platform accommodates B cell screening from both human donors and immunized animal species, such as mice, rabbits, and non-human primates. This flexibility allows for customized antibody solutions, whether your project requires human-derived antibodies or antibodies from alternative sources.

5. Direct Access to In Vivo Maturation
   By isolating naturally occurring antibodies, we obtain fully functional antibodies that have undergone in vivo maturation. These antibodies often exhibit higher binding affinity, durability, and potency, essential for therapeutic applications.

Our Single B Cell Screening Workflow

1. Antigen Design and Immunization
   We begin with antigen design to ensure a strong immune response. After immunization of animal models (e.g., mouse, rabbit) or through human sample collection (e.g., PBMCs from immunized or infected individuals), we isolate B cells for screening.

2. Single B Cell Isolation
   Using techniques like fluorescence-activated cell sorting (FACS) or microfluidic platforms, we isolate individual B cells that bind specifically to your antigen of interest. This precision ensures only high-affinity B cells are selected for downstream processes.

3. Antibody Gene Amplification and Cloning
   After B cell sorting, we amplify the antibody gene sequences using RT-PCR to recover both heavy and light chain genes. These genes are then cloned into expression vectors, facilitating recombinant production in mammalian or other expression systems.

4. Recombinant Antibody Expression and Screening
   We express the recombinant antibodies and conduct rigorous screening through ELISA, flow cytometry, or other functional assays to confirm binding affinity, specificity, and activity.

5. Antibody Production and Purification
   After identifying high-performing antibodies, we scale up production and purify them using protein A/G chromatography and other purification methods. Our purified antibodies are ready for immediate application or further development.

6. Characterization and Quality Control
   All antibodies undergo thorough quality control and characterization, including isotyping, binding affinity analysis, and functional validation. This ensures they meet the highest standards of purity, stability, and effectiveness.

Applications of Single B Cell Screening

• Therapeutic Antibody Discovery
  Discover high-affinity, therapeutic-grade antibodies for targeted applications, including cancer immunotherapy, infectious disease treatment, and autoimmune disease management. Our service expedites the discovery of lead candidates by providing biologically relevant antibodies from immunized sources.

• Vaccine Development
  Identify antibodies with neutralizing capabilities against pathogens, aiding in vaccine development and immune response studies. Single B cell screening accelerates the identification of antibodies that recognize critical epitopes required for pathogen neutralization.

• Diagnostic Antibody Generation
  Generate antibodies for diagnostic assays such as ELISA, flow cytometry, and lateral flow tests. Single B cell screening allows rapid production of antibodies with high specificity, increasing the accuracy of biomarker detection or pathogen identification.

• Rare Epitope Identification
  By targeting rare or unique epitopes, single B cell screening enables the development of antibodies against challenging antigens, including cancer markers, viral proteins, and modified antigens.

Advantages of Our Single B Cell Screening Service

• Rapid Turnaround
  By isolating and cloning antibodies directly from B cells, single B cell screening provides high-quality antibodies in weeks rather than months.

• High Sensitivity and Specificity
  Screening at the single-cell level ensures only high-specificity, high-affinity antibodies are selected, ideal for critical applications like diagnostics and therapeutics.

• Fully Functional Antibodies
  Directly isolating B cells producing antibodies ensures that the resulting antibodies are naturally matured and functional, ready for downstream applications without further optimization.

Why Partner with Us?

• Advanced Technologies: We use cutting-edge FACS and single-cell sorting techniques, ensuring precision and efficiency in isolating antigen-specific B cells.
• Experienced Team: Our team of immunologists and molecular biologists is highly skilled in antibody screening and production, ensuring your project’s success.
• Customized Solutions: We tailor every aspect of our service to meet your specific needs, from antigen design to final antibody production.
• Reliable Results: With our rigorous quality control, we ensure that every antibody meets the highest standards of performance and reproducibility.

Contact us today to learn how our Single B Cell Screening Service can accelerate your antibody discovery and development process with high-affinity, high-specificity antibodies tailored to your research, diagnostic, or therapeutic needs.