Our Antibody Humanization Service provides a precise, efficient approach to minimizing the immunogenicity of non-human antibodies for therapeutic applications. Using advanced molecular engineering techniques, we modify rodent, rabbit, and other non-human antibodies, replacing non-human components with human sequences to retain binding affinity, specificity, and functionality. This process enables the safe use of these antibodies in humans, making them ideal for therapeutic use in a wide range of clinical applications.

Why Choose Antibody Humanization?

1. Reduced Immunogenicity
   By replacing non-human antibody regions with human equivalents, antibody humanization minimizes the risk of immune responses when administered to patients. This reduction in immunogenicity is critical for therapies requiring long-term antibody use.

2. Preservation of Binding Affinity and Specificity
   Our humanization process maintains the original antibody’s antigen-binding properties, ensuring that the modified antibody retains the same high specificity and affinity as the original non-human version.

3. Improved Safety and Efficacy for Therapeutic Use
   Humanized antibodies are better tolerated in the human body, with improved pharmacokinetics and reduced immunogenic responses, making them more effective for therapeutic applications such as cancer immunotherapy, autoimmune treatment, and infectious disease management.

4. Broad Compatibility Across Species
   Our humanization service is suitable for a variety of non-human antibodies, including those derived from rodents, rabbits, and other animal sources, offering a versatile solution to antibody development.

5. Accelerated Path to Clinical Development
   Humanized antibodies meet regulatory standards more readily than non-human-derived antibodies, simplifying the path to clinical trials and regulatory approval. This service accelerates timelines for therapeutic development and clinical testing.

Our Antibody Humanization Process

1. Sequence Analysis and Framework Selection
   We start by analyzing the variable regions of your non-human antibody, focusing on complementarity-determining regions (CDRs) essential for antigen binding. Our team selects the most suitable human framework sequences based on homology and compatibility, ensuring minimal disruption to the antibody’s functionality.

2. CDR Grafting
   The CDRs from the non-human antibody are grafted onto the selected human framework. Our experts carefully optimize this process to preserve the binding characteristics of the original antibody, adjusting only where necessary to enhance stability and binding affinity.

3. Back Mutation Optimization
   To further improve binding affinity and functionality, we perform back mutations by reintroducing essential amino acids from the original non-human antibody into the humanized version. This helps to ensure the humanized antibody retains the binding strength and specificity of the original antibody.

4. Molecular Modeling and Validation
   Using advanced molecular modeling tools, we evaluate the structural integrity and binding capacity of the humanized antibody. This computational analysis allows us to refine the humanized sequence for stability and functionality before laboratory testing.

5. Expression and Production
   The humanized antibody genes are synthesized and expressed in mammalian cell systems, providing a recombinant form of the antibody for further testing. This step ensures that the humanized antibody is fully functional and ready for rigorous assessment.

6. In Vitro Testing and Validation
   We conduct binding assays, such as ELISA, flow cytometry, and surface plasmon resonance (SPR), to confirm that the humanized antibody retains its affinity and specificity. Functional assays may also be performed to validate activity against the target antigen.

7. Quality Control and Delivery
   After validation, we conduct extensive quality control to ensure the antibody meets high standards for purity, stability, and activity. We then deliver the fully humanized antibody in your preferred format and concentration, ready for further research or clinical development.

Applications of Antibody Humanization

• Therapeutic Development
  Humanized antibodies are essential for developing treatments for diseases such as cancer, autoimmune disorders, and infectious diseases. Our service provides antibodies optimized for clinical use, with minimized immunogenicity for better patient outcomes.

• Diagnostic Assays
  For diagnostic applications requiring safe and high-affinity antibodies, humanized antibodies ensure compatibility with human samples while maintaining accuracy and specificity, crucial for biomarker detection and disease monitoring.

• Biomarker Research
  Humanized antibodies are invaluable tools in translational research, enabling accurate and reproducible biomarker analysis. They are especially useful for disease progression studies and therapeutic target validation.

Advantages of Our Antibody Humanization Service

• High Retention of Binding Affinity
  We carefully engineer humanized antibodies to retain high binding affinity and specificity, crucial for both therapeutic and diagnostic applications.

• Rigorous Quality Control
  Every humanized antibody undergoes extensive testing to ensure it meets the highest standards for stability, purity, and activity, delivering high-quality results for your application.

• Comprehensive Support and Expertise
  Our team of scientists provides end-to-end support, from the initial antibody design to final validation, helping you navigate every step of the humanization process.

• Fast Turnaround
  Our streamlined workflow and expertise in antibody engineering enable us to deliver high-quality humanized antibodies rapidly, supporting your project’s timelines and goals.

Why Partner with Us?

• Advanced Technologies: We use cutting-edge techniques for sequence analysis, molecular modeling, and CDR grafting, ensuring accuracy and precision in each project.
• Experienced Team: Our scientists specialize in antibody engineering and humanization, with extensive experience in therapeutic development and research applications.
• Customized Solutions: We tailor our humanization process to meet the specific needs of your project, offering flexibility in antibody format and functionality.
• Regulatory-Ready Antibodies: Our humanized antibodies are produced with the highest quality standards, meeting regulatory expectations for therapeutic use.

Contact us today to discuss how our Antibody Humanization Service can transform your non-human antibodies into optimized human-compatible candidates. We’re here to support your path to safe, effective, and high-quality antibody-based therapeutics.